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[Optimization of the biochemical analysis of several metabolic products of protein turnover in pulmonary microsomes].
The use of mouse enzyme-secreting microsomes as an alternative to obtain lung samples from mammals is described. The metabolic rates of tyrosine and of valine, leucine and phenylalanine are faster in the lungs than in liver, which suggests that the proteins derived from them are regulated in tissues other than the liver. The lung microsomal fraction released 95-99% of the proteins tyrosine and valine, 97% of leucine and phenylalanine. The analytical methods developed are suitable for determining either the relative rates of synthesis or degradation of proteins.The Endonuclease V of vaccinia virus and host cell DNA.
The reaction of purified vaccinia virus endonuclease with genomic DNA, linear or supercoiled, was studied. DNAase V was found to exhibit a weak endonucleolytic activity against a relaxed circular template, but a strong exonuclease activity against ssDNA. Degradation of the template was followed by loss of the adenine at one terminus and a decrease in the rate of the continuous net migration of the oligonucleotide released from the template. Under certain conditions, endonuclease V degraded mononucleotide 3′ overhangs into double-strand breaks, but this was accompanied by a decrease in the extent of degradation. No effect was seen on an RFI-containing oligonucleotide.Q:
I have to build a chat application that shows a list of friends’ names. Each friend is shown as a , with his/her status on the left and their name on the right. Clicking on a friend’s name results in a ‘+’ button with the friend’s status being updated to “online”.
There are some problems :
The profile may be firstly loaded into